The transcriptional response to unilateral nephrectomy with and without ischemia-reperfusion injury. Rattus norvegicus

Transcriptome analysis was done after warm renal ischemia-reperfusion injury (IRI) in a rat model. Earlier studies have shown a protective effect of prior unilateral nephrectomy (UNx) against IRI in the remaining, contralateral kidney compared to a non-neprectomized control group. We aimed at identifying the underlying molecular mechanisms. We used the Affymetrix Clariom D array (formerly known as RTA 1.0 st.) Array data was processed in the Affymetrix Console Software. Overall design: We analysed kidney tissue (left) from male Wistar rats. Number of animals from each group, n = 3. Wistar rats were randomized to either UNx or sham UNx immediately prior to 37 minutes of unilateral renal artery clamping or sham operation under sevoflurane anesthesia. Groups: sham (2 normal kidneys), UNx (1 remaining kidney), IR (1 ischemic kidney, 1 normal kidney), IR+UNx (1 ischemic kidney). MRI was performed 24 hours after reperfusion. Blood and renal tissue were harvested. RNA was isolated for microarray analysis.