Hippocampal transcriptome analysis of glucocorticoid enhanced memory formation [RNA-seq]

Methods: Adult male rats received 3 minutes of object exploration training or no training either with a 3.0mg.kg post-training corticosterone or vehicle injection to study the molecular effects underlying enhanced memory formation. Hippocampi were dissected 3 hours after injection and snap-frozen. Subsequently, total RNA was isolated and send for 100bp paired-end sequencing by BGI.实验方法：为探究记忆形成增强背后的分子效应，对成年雄性大鼠开展3分钟物体探索训练或不进行训练，并于训练后注射3.0mg/kg的皮质酮（corticosterone）或溶媒（vehicle）；于注射后3小时摘除大鼠海马体并快速冷冻保存；随后提取总RNA，交由华大基因（BGI）开展100bp双端测序。