Eco–enzymatic stoichiometry unveils resource limitations in soil microorganisms during subtropical vegetation restoration

Eco–enzymatic stoichiometry provides insights into how soil microorganisms allocate resources to meet their energy and nutrient needs. Subtropical ecosystems are known to be strongly resource-limited, particularly for phosphorus (P) limitation. Vegetation restoration usually sustainably further alters the relative availability of soil carbon (C), nitrogen (N), and P. However, the patterns of microbial nutrient limitation and their drivers along vegetation restoration remain largely unclear.
Here, using eco–enzymatic stoichiometry modeling, we investigated soil microbial resource limitations and identified primary factors driving them across five stages of vegetation restoration [grassland (GL), shrubland (SL), early–, mid–, and late–forest (EF, MF, and LF)] in subtropical China.
β–1,4–glucosidase (BG), β–N–acetyl glucosaminidase (NAG), and acid phosphatase (ACP) activities in forest stages (EF, MF, and LF) were significantly higher than the GL and SL stages. Cellobiohydrolase (CBH) and ..., , # Data from: Eco–enzymatic stoichiometry unveils resource limitations in soil microorganisms during subtropical vegetation restoration

Dataset DOI: [10.5061/dryad.r4xgxd2rp](10.5061/dryad.r4xgxd2rp)

## Description of the data and file structure

Wan, J., Zhang, R., Jiang W.T., Chen, Y.F., Yang, J., Hu, B.A., & Ouyang, S. (2025). Data from: Eco-enzymatic stoichiometry unveils resource limitations in soil microorganisms during subtropical vegetation restoration. Journal of Applied Ecology

### Files and variables

#### File: Raw_data.xlsx

**Description:** Raw data belonging to:

Wan, J., Zhang, R., Jiang W.T., Chen, Y.F., Yang, J., Hu, B.A., & Ouyang, S. (2025). Data from: Eco-enzymatic stoichiometry unveils resource limitations in soil microorganisms during subtropical vegetation restoration. Journal of Applied Ecology

#### Data for Physicochemical properties

| Entry | Unit | Explanation ...,