Microarray was used to detect the expression of lncRNAs and mRNAs in INS-1 cells treated with GLP-1RA Geniposide for 24 hours.

Studies have shown that long noncoding RNAs (lncRNAs) can be widely involved in various physiological and pathological processes. In recent years, there have been many studies on GLP-1 receptor agonists (GLP-1RA) regulating islet β cells function and mass of type 2 diabetes (T2DM) patients. However, the function of lncRNAs in this process have not been fully elucidated. In this study, lncRNA microarray was used to identify the differently expressed (DE) lncRNAs and mRNAs in β cells exposed to Geniposide, which is a GLP-1RA. 308 lncRNAs and 128 mRNAs were detected with a set filter fold change≥1.5 and P-value<0.05. Gene Ontology and KEGG pathway analysis were performed to assess the underlying functions of DE mRNAs. Co-expression network of DE lncRNAs and mRNAs was constructed based on Pearson coefficient of expression level. And hub mRNAs were selected through String database and Cytoscape plugin Cytohubba. Additionally, a ceRNA network was constructed among the co-expressed lncRNAs and hub mRNAs. This study reveals key mRNAs involved in the regulation of GLP-1RA on β cells function and mass. More importantly, screening out lncRNAs that play an importance regulatory role in this process. This original research could provide valuable information for the further investigation between lncRNAs and GLP-AR in the protection of β cells.已有研究证实，长链非编码RNA（long noncoding RNAs，lncRNAs）广泛参与多种生理及病理过程。近年来，诸多研究围绕胰高血糖素样肽-1受体激动剂（GLP-1 receptor agonists，GLP-1RA）对2型糖尿病（type 2 diabetes，T2DM）患者胰岛β细胞功能与数量的调控作用展开。然而，长链非编码RNA在该调控过程中的具体功能尚未完全阐明。本研究采用长链非编码RNA基因芯片，对经GLP-1RA类化合物京尼平苷（Geniposide）处理的β细胞中的差异表达（differently expressed，DE）长链非编码RNA与信使RNA（messenger RNA，mRNA）进行筛选鉴定。按照预设筛选条件（折叠变化≥1.5且P值<0.05），共鉴定得到308个差异表达长链非编码RNA与128个差异表达信使RNA。本研究通过基因本体（Gene Ontology）与KEGG通路（KEGG pathway）富集分析，对差异表达信使RNA的潜在功能进行评估。基于基因表达水平的皮尔逊相关系数，构建差异表达长链非编码RNA与信使RNA的共表达网络，并通过STRING数据库与Cytoscape插件CytoHubba筛选得到核心信使RNA。此外，在共表达的长链非编码RNA与核心信使RNA之间构建了内源竞争RNA（competing endogenous RNA，ceRNA）网络。本研究明确了参与胰高血糖素样肽-1受体激动剂调控胰岛β细胞功能与数量的核心信使RNA，更为重要的是筛选出在该调控过程中发挥关键作用的长链非编码RNA。本原创研究可为后续探索长链非编码RNA与GLP-AR在保护胰岛β细胞中的相关机制提供有价值的参考依据。