Rapamycin effect on gene expression: dependence on c-Myc

This experiment utilized rat fibroblasts that are wild-type for c-Myc (TGR-1 cells) and null for c-Myc (HO15.19 cells). Both were treated treated with rapamycin for 24 hr (comparison group, vehicle control).本实验采用了两种大鼠成纤维细胞：分别为c-Myc野生型TGR-1细胞与c-Myc敲除型HO15.19细胞。将两类细胞均以雷帕霉素处理24小时，同时设置溶剂对照组作为对照。