RNA sequencing of Met versus non-Met amplified recurrent mouse mammary tumors

We performed RNA-seq on tumors from orthotropic injections of cells from the MTB;TAN model. We aimed to more broadly compare gene expression patterns between primary tumors, Met-amplified recurrent tumors, and non-Met amplified recurrent tumors. Principal components analysis revealed that these tumors clustered by group, indicating that each of these cohorts had a distinct gene expression pattern. Principal component 1 (PC1) efficiently separated primary tumors from recurrent tumors, while PC2 partly separated Met-amplified from non-amplified tumors. Taken together, this suggests that non-Met amplified recurrent tumors have a unique gene expression profile.