eDNA‑based detection of the invasive crayfish Pacifastacus leniusculus in streams with a LAMP assay using dependent replicates to gain higher sensitivity

<p>The records in this dataset were collected and evaluated for the following publication:
Porco, D., Hermant, S., Purnomo, C.A. et al. eDNA-based detection of the invasive crayfish Pacifastacus leniusculus in streams with a LAMP assay using dependent replicates to gain higher sensitivity. Sci Rep 12, 6553 (2022). https://doi.org/10.1038/s41598-022-10545-w</p>
<p>Abstract of the publication:
LAMP assays are becoming increasingly popular in the feld of invasive species detection but are still underused in eDNA-based monitoring. Here, we propose a LAMP assay designed to detect the North American crayfsh species Pacifastacus leniusculus in water samples from streams. The presence of P. leniusculus was detected through this new LAMP assay in all but one of the nine sites sampled. No correlation was found between ddPCR absolute concentration measurements and the number of LAMP-positive technical replicates. However, we showed that using dependent technical replicates could signifcantly enhance the detection sensitivity of the LAMP assay. Applied to other assays, it could improve sensitivity and thus allow for a more efcient use of eDNA-based LAMP assays for invasive species detection in aquatic ecosystems.</p>

<p>本数据集之记录系为以下出版物所收集与评估：</p><p>Porco, D., Hermant, S., Purnomo, C.A. 等. 基于eDNA的入侵性螯虾Pacifastacus leniusculus在溪流中的LAMP检测及其依赖重复试验以提高灵敏度的研究. 科学报告 12, 6553 (2022). https://doi.org/10.1038/s41598-022-10545-w</p><p>论文摘要：</p><p>环介导等温扩增（LAMP）技术在入侵物种检测领域日益受到青睐，但其在基于eDNA的监测中的应用尚显不足。本研究提出一种专为检测来自溪流水样的北美螯虾物种Pacifastacus leniusculus而设计的LAMP检测方法。通过该新LAMP检测，在九个采样点中除一个外均检测到了P. leniusculus的存在。未发现ddPCR绝对浓度测量值与LAMP阳性技术重复次数之间存在相关性。然而，我们证实使用依赖性技术重复试验能够显著提升LAMP检测的灵敏度。将该技术应用于其他检测方法，有望提高检测灵敏度，从而实现基于eDNA的LAMP检测在水生生态系统入侵物种监测中的更高效应用。</p>