MicroCT of stained bovine IVD quarter segments after 2 weeks incubation

Visualising the 3D microstructure of stained and native intervertebral discs using X-ray microtomography

C.M. Disney 1,2, K. Madi 3, A.J. Bodey 4, P.D. Lee 3, J.A. Hoyland 2,5, M.J. Sherratt 2

1 Centre for Doctoral Training in Regenerative Medicine, 2 Division of Cell Matrix Biology and Regenerative Medicine, 3 School of Materials, University of Manchester, UK.

4 Diamond Light Source, Harwell Science and Innovation Campus, Oxfordshire, UK.

5 NIHR Manchester Biomedical Research Centre, Central Manchester University Hospitals NHS Foundation Trust, Manchester Academic Health Science Centre, UK.

A comparison of three stains; iodine potassium iodide (I2KI), phosphomolybdic acid (PMA) and phosphotungstic acid (PTA). Chemically fixed (10% formal saline) quarter segments of bovine tail intervertebral disc were incubated for two weeks before being imaged by laboratory microCT. Reconstruction software (Phoenix dato s|x2 reconstruction) was used to generate 3D datasets from the projections. This reconstructed data relates to figure 3 and is saved as 8-bit .raw with image dimensions given in the file name.

运用X射线微断层扫描技术可视化染色及原态椎间盘的3D微观结构

C.M. Disney 1,2, K. Madi 3, A.J. Bodey 4, P.D. Lee 3, J.A. Hoyland 2,5, M.J. Sherratt 2

1再生医学博士研究生培养中心，2细胞基质生物学与再生医学系，3材料学院，曼彻斯特大学，英国。
4Diamond Light Source，哈雷尔科学创新园区，牛津郡，英国。
5NIHR曼彻斯特生物医学研究中心，中央曼彻斯特大学医院NHS基金会信托，曼彻斯特学术卫生科学中心，英国。

本研究对比了三种染色剂：碘化钾碘（I2KI）、磷钼酸（PMA）和磷钨酸（PTA）。以10%的福尔马林进行化学固定的牛尾椎间盘四分之一段在孵化两周后，通过实验室微CT进行成像。使用重建软件（Phoenix dato s|x2重建）从投影生成3D数据集。此重建数据与图3相关，并以8位.raw格式保存，图像尺寸信息包含于文件名中。