Alloreactive memory CD4 T cells promote transplant rejection by engaging DCs to induce innate inflammation and CD8 T cell priming

Alloreactive memory t cells have been implicated as central drivers of transplant rejection. In our study we find that alloreactive memory T cells can interact with and engage Dendritic cells(DCs) Alloantigen-specific memory T cells were generated in vitro by co-culturing naïve CD4T cells from BALB/c mice with splenic DCs derived from B6 mice( by negatively sorted to remove T,B,NK and macrophages) for 5 days and then rested with IL2 for 2 days. Syngeneic memory T cells were similarly derived from B6 mice and polarized in vitro. Alloreactive or syngeneic memory T cells were then co-cultured for 3 hours with B6 splenic DCs. CD11b+CD11c+ cells were sorted away from T cells after 3 hours and lysed for RNA seq analysis. mRNA profiles of B6 splenic DCs stimulated with syngeneic or allogeneic effector memory T cells for 3 hours by Illumina sequencing in triplicate.