scRNA-seq of embryonic skin development (developmental time series in full-thickness dorsal skin of mouse embryos)

In this study, we analysed early embryonic skin development (mus musculus; C57BL/6J) at the transcriptional level. Major questions concerned the cell type composition of early embryonic skin, and the emergence of transcriptional heterogeneity among epithelial and stromal precursor cells. Cells were isolated from embryonic dorsal skin and randomly sequenced (scRNA-Seq using 10X Genomics v2) without any cell sorting. Data from three embryonic time points (E12.5, E13.5, and E14.5) was integrated and compared to obtain a better understanding of the dynamics of early skin development.本研究在转录组水平上分析了小家鼠（Mus musculus）C57BL/6J品系的早期胚胎皮肤发育进程。本研究的核心问题聚焦于早期胚胎皮肤的细胞类型组成，以及上皮与基质前体细胞群体中转录异质性的产生过程。研究人员从胚胎背部皮肤分离细胞，未进行任何细胞分选步骤，采用10X Genomics V2平台开展单细胞RNA测序（scRNA-Seq）。本研究整合三个胚胎时间点（E12.5、E13.5与E14.5）的测序数据并进行对比分析，以更深入地解析早期皮肤发育的动态变化规律。