Single-cell RNA-seq profiles of murine sarcoma tumors with vehicle and digoxin treatments

Digoxin, a cardiac glycoside widely used in humans, acts through disruption to central carbon metabolism via on target inhibition of the Na+/K+ ATPase. Acute Digoxin treatment remodels the tumor microenvironment, leading to cell-type specific transcriptional reprogramming of metabolic processes. Overall design: We treated murine allograft sarcoma model with vehicle or 2 mg/kg digoxin (using two mice per group) every 24 hours and collected tumors after the fourth dose.