Quantitative measurements of adipocyte-related gene expression in aortic perivascular adipose from GPER0 and control mice.

Adipose was collected and analyzed from animals with monogenic (GPER0) obesity and compared to lean WT controls (n = 5/group). Expression levels of mRNA were calculated based on the 2−ΔΔCT method and expressed as arbitrary units. GAPDH served as the housekeeping control.