Eeffect of Plk1 inhibitor on the gene expression profile FANCD2 wild type and mutant cell lines

Fanconi anemia (FA) is a rare disease passed down through families (inherited) that mainly affects the bone marrow. The loss-of-function of FA genes has been shown to be associated with predisposition of cancer. FANCD2 is one of the two genes form the FA core complex, which is the hub of FA signaling pathway. So far, there is limited ways for the intervention of FA, excepting for bone marrow transplantation. Synthetic lethality describes a situation in which mutations (changes) in two genes together result in cell death, but a mutation in either gene alone does not. We identified the synthetic lethal relationship between PLK1 and FANCD2. To elucidate the molecular mechanism, we performed RNA sequencing with PLK1 inhibitor treated FANCD2 wild type (D2) and mutant (PD20) cell lines. The differentially expressed genes were identified through bioinformatics analysis. The information obtained from this study would be valuable for the development of new strategies and drugs for the treatment of FA.