Comparison(1) of the three plasmid purification methods.

(1)This comparison was obtained based on retail price for each reagent because commercial kit for this method is not currently available.(2)The time required for each method is the period necessary to get purified plasmid DNA starting with a 200 ml E.coli culture. For Qiagen Maxiprep kit (Cat. No.: 12163) [14], time was calculated from the manufacture's instructions.(3)The cost of Qiagen Maxiprep kit (Cat. No.12163) is $ 409 per 25 preparations so that the cost of each preparation is $16.36. This cost calculation doesn't include any additional laboratory materials that are required.(4)The cost includes 5 glass syringe filters ($ 1 each), a 60 ml syringe ($ 1) and the required reagents.