Small RNA profiling of differentiated and de-differentiated vascular smooth muscle cell

We report the application of small RNA sequencing for high-throughput profiling of small RNA under 75 bp in vascular smooth muscle cell. By a reading depth of 30M and single stranded sequencing, we generated the small RNA signature on differentiated and de-differentiated vascular smooth muscle cell induced by PDGF-BB and H3K4me2 editing. We found that PDGF-BB and H3K4me2 editing induced de-differentiation modulated miRNA profile significantly, which was demonstrated at least in part responsible for modulated vascular smooth muscle cell phenotype.本研究报道了小RNA测序（small RNA sequencing）在血管平滑肌细胞（vascular smooth muscle cell）中针对75 bp以下小RNA的高通量谱分析中的应用。本研究通过30百万条测序读段的测序深度及单链测序策略，构建了由血小板衍生生长因子-BB（PDGF-BB）与组蛋白H3赖氨酸4二甲基化（H3K4me2）编辑诱导的分化型与去分化型血管平滑肌细胞的小RNA特征谱。研究发现，PDGF-BB与H3K4me2编辑诱导的去分化过程可显著调控微小RNA（miRNA）表达谱，该调控作用至少部分被证实与血管平滑肌细胞表型的改变相关。