Transcription profiling of embryonic basal ganglia and cortex in wild type and Dlx1/2 mutant mice to identify genes that are downstream of Dlx1/2 functions

The Dlx homeodomain transcription factors are implicated in regulating the function of inhibitory neurons; therefore understanding their functions will provide insights into disorders such as epilepsy, mental retardation, autism and cerebral palsy. Comparing gene expression in the embryonic basal ganglia and cortex in wild type and dlx1/2 mutant mice will provide information regarding the types of genes that are downstream of Dlx1/2 function. Perform gene array analyses in duplicate or triplicate; compare expression profile of total RNA from wild type and dlx1/2 telencephalons. I am sending separate samples of the Basal Ganglia (BG) and cortex. I want to perform gene expression comparison between: 1) wild type and dlx1/2 basal ganglia (BG) 2) wild typee and dlx1/2 cortex (ctx). Age of all mice has been indicated as 14 days to satisfy system requirements, however all mice are embryonic day 14.5.Dlx同源域转录因子（Dlx homeodomain transcription factors）参与调控抑制性神经元的功能，因此解析其功能机制将为癫痫、智力障碍、孤独症及脑瘫等神经系统疾病的研究提供新视角。通过对比野生型与dlx1/2突变小鼠胚胎期基底神经节及大脑皮层的基因表达谱，可明确受Dlx1/2调控的下游靶基因类别。本实验将进行两次或三次重复的基因芯片分析，对比野生型与dlx1/2突变小鼠端脑总RNA的表达谱。本次将单独寄送基底神经节（Basal Ganglia, BG）与大脑皮层样本，拟开展两组基因表达对比实验：1）野生型与dlx1/2突变小鼠基底神经节（BG）；2）野生型与dlx1/2突变小鼠大脑皮层（ctx）。为满足实验系统要求，所有小鼠样本标注为14日龄，但实际均为胚胎14.5天龄的小鼠。