Expanding and improving analyses of nucleotide recoding RNA-seq experiments with the EZbakR suite
收藏资源简介:
Data necessary to reproduce figures in manuscript titled "Expanding and improving analyses of nucleotide recoding RNA-seq experiments with the EZbakR suite". Includes: Compressed arrow dataset used to produce Figure S6 (subtlseq_dataset.tar) Annotations used to process data (Hs_ensembl_lvl1_and_2.gtf and Hs_ensembl.gtf) Processed data to produce Figures 3C and S2 (cB_ensembl_totRNAsubtlseq.csv.gz and cB_ensemblLvl1and2_totRNAsubtlseq.csv.gz, respectively). Also includes supplemental tables of: List of genes producing transcripts predicting to undergo nuclear decay (PUNDs; Supplemental_Table_PUNDs.csv) Estimates for mature RNA synthesis, nuclear degradation, nuclear export, and cytoplasmic degradation rate constants from Ietswaart et al., 2024 total-cytoplasmic-nuclear TimeLapse-seq dataset (Supplemental_Table_NucCytoEsts.csv) Estimates for premature RNA synthesis, premature RNA processing, and mature RNA degreadation obtained from EZbakR analysis of Ietswaart et al., 2024 total RNA TimeLapse-seq dataset (Supplemental_Table_PtoMests.csv). Scripts to reproduce figures can be found at: https://github.com/isaacvock/EZbakRsuite_paper_code



