Label-free shotgun proteomics of tmRNA substrates enriched by immunoprecipitation
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https://www.omicsdi.org/dataset/pride/PXD023973
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We used a combination of genetic and proteomic approaches to characterize tmRNA (ssrA) activity in the genome-reduced bacterium Mycoplasma pneumoniae. For this, we generated tmRNA mutants encoding a tag resistant to proteolysis. Endogenous protein tagging by the mutant tmRNA gene (ssrAmk) was then examined by immunoprecipitation (IP) enrichment followed by LC-MS/MS analysis.
创建时间:
2021-08-23



