Impact of RNA Lipid Particle Aggregates on the Tumor Microenvironment in a Murine Brain Tumor Model

Mice bearing KR158b-luc intracranial tumors underwent treatment with one of the following: lipid particles, silenced RNA-loaded lipid particle aggregates (modRNA-LPA), or unsilenced RNA-loaded lipid particle aggregates. Twenty-four hours post-treatment, the tumor tissues were preserved in RNAlater, followed by RNA extraction. The samples were then dispatched to Novogene for library preparation via Poly(A) capture. Sequencing was conducted on the Illumina NovaSeq 6000 platform, utilizing a paired-end 150 bp sequencing approach. The RNA used for treatment was synthesized from total RNA extracted from the parental KR158b-luc cell line. A cDNA library was generated using RT-PCR prior to its use in in vitro transcription (IVT) reactions, followed by RNA cleanup to obtain purified mRNA antigens. The unsilenced RNA was produced using the ThermoFisher Scientific IVT kit (Cat. AM1344), while the silenced RNA was generated with the CellScript IVT kit (Cat. C-ICTAMY110510).