Characterization of Neuron Action Potential Triggering Using Sine Wave Electrostimulation at Varying Frequencies_part4

This experimental series aimed to characterize the triggering of action potentials (APs) in neurons using sine waves and modulated sine waves at varying frequencies. The experiments were conducted on E18 Sprague Dawley rat hippocampal neurons (BrainBits, Springfield, IL), employing the voltage-sensitive dye FluoVolt (Thermo Fisher Scientific, Waltham, MA) as the AP reporter.