Comparing transcrional changes in Plasmodium yoelii infected to uninfected primary hepatocytes

GFP expressing Plasmodium yoelii (Py-GFP) infected, or naïve cultured primary hepatocytes were FACS sorted and re-suspended in TRIzol and RNA purified with RNAeasy kit (Qiagen).RNA was assessed for purity and quality using an Agilent 2100 Bioanalyzer and transcriptome analysis performed on Affymetrix GeneChip Mouse Transcriptome array 1.0. Data output was analyzed and visualized using Affymetrix Expression Console 4.0. Infected hepatocytes were sorted based on the fluorescence signal from infecting Plasmodium yoelii (GFP) and compared to uninfected, sorted hepatocytes from an uninfected culture