Hs27-CRISPRa-TFs

Processed dataset of all transcription factor CRISPRa perturbations within Hs27 cells (fibroblasts) from: Comprehensive transcription factor perturbations recapitulate fibroblast transcriptional states Southard KM, Ardy RC, Tang A, O'Sullivan DD, Metzner E, Guruvayurappan K, Norman TM. Comprehensive transcription factor perturbations recapitulate fibroblast transcriptional states. bioRxiv [Preprint]. 2024 Aug 3:2024.07.31.606073. doi: 10.1101/2024.07.31.606073. PMID: 39131349; PMCID: PMC11312553. in press at Nature Genetics Raw fastq files available at SRA: PRJNA1108254 This record contains aggregated, quality control filtered, and processed outputs downstream of cellranger including. 1) guide umis per cell: fibroblast_CRISPRa_aggr_total_guide_umis.h5 2) final qc filtered population with multiplets: fibroblast_CRISPRa_final_pop.h5ad 3) filtered singlets, processed with scanpy.pp.normalized_total and scanpy.pp.log1p. Raw counts saved in layer['counts']: fibroblast_CRISPRa_final_pop_singlets_normalized_log1p.h5ad 4) "mean" population constructed from the full regression model from overloaded droplets: fibroblast_CRISPRa_mean_pop.h5ad