In vivo lipid lowering leads to reduced aortic root CD68+ area, independent of diet switch.

Reversa mice were fed a Western diet for 16 weeks. One group of animals was sacrificed at 16 weeks as the baseline group. The rest of the mice were switched to chow diet and assigned to two groups, vehicle and Mttp-inactivated; animals within these two groups received injections of saline or poly I:C, respectively (see Methods). Animals were sacrificed on day seven or day 14 after the injections, and aortic roots were sectioned, immunostained for CD68, and the CD68+ areas were measured (see Methods). Measurements are summarized as mean ± SE.In vivo lipid lowering leads to reduced aortic root CD68+ area, independent of diet switch.