Treatment of human hepatoma and primary human hepatocyte cultures (PHH) with fatty acids and/or fatty acids and TNFa. Treatment of human hepatoma and primary human hepatocyte cultures (PHH) with fatty acids and/or fatty acids and TNFa

Treatment of the human hepatoma cell lines HepG2, HUH7 and PHH with a mixture of fatty acids caused heaptic steatosis. The combined lipid and TNFa treatment mimics inflammation. Cells were harvested and processed for microarray analysis Overall design: Three independent experiments were performed with the human hepatoma cell line HepG2, HUH7 and primary human hepatocytes (PHH)