Comparative analysis of viral genome detection via real-time RT-PCR. Mean Cq values from duplicate runs.
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RABV: Rabies virus; ;EBLV: European Bat Lyssavirus;neg.: negative control; −: negative result; #: cross-reactivitiy with other Lyssavirus species; ?: doubtful result;*doubtful result was retested; i.h.: in-house assay; dilution series (0), (I), (II), (III); 100, 10−1, 10−2, 10−3; mod.: assay modified; r: RABV-specific detection; e1: EBLV-1 specific; e1+2: EBLV-1+−2 specific; r13, r14, r13/14: R13, R14, duplex R13/14 assay by [17]; fn: false negative results; exp: expected negative results from previous publication; ts: two-step systems; no duplicates for assays D2, D3 and M; 2) Orlowska et al., 2008 [22].
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2015-12-02
