Phosphorus status data from the effects of alendronate, daidzein, tempeh, and probiotics in a postmenopausal rat model with calcium deficit

This dataset originates from a controlled preclinical study investigating the influence of alendronate, daidzein, tempeh, and probiotics on phosphorus metabolism in a postmenopausal rat model. The experiment was designed to assess how these dietary interventions—administered individually or in combination—affect phosphorus intake, absorption, and tissue distribution in ovariectomized (OVX) and sham-operated (S) female rats over a six-week intervention period.All procedures were conducted in accordance with ethical standards for animal experimentation, approved by the Lokalna Komisja Etyczna (Poznań, Poland), under registration number 21/2021. The experimental design comprised several dietary groups: a sham-operated control (S), an ovariectomized control (O), and treatment groups fed with alendronate (OB), daidzein (OD), tempeh (OT), probiotic (OL), combination of daidzein and probiotic (ODL), and combination of tempeh and probiotic (OTL). Diets were formulated based on the AIN-93M standard, with specific modifications to incorporate the designated bioactive compounds.The dataset includes detailed records of phosphorus intake, phosphorus concentrations in the experimental diets, and food consumption across all groups. Following the intervention, biological samples—including feces and various organs (spleen, femur, heart, liver, kidney, and pancreas)—were collected to quantify total phosphorus content using the ammonium molybdate–vanadate colorimetric method.Collectively, this dataset provides a comprehensive overview of how dietary phytoestrogens (daidzein and tempeh) and probiotics modulate phosphorus homeostasis and tissue-specific deposition patterns in a postmenopausal rat model. The data serve as a foundation for exploring nutrient–bioactive interactions relevant to mineral metabolism, gut–bone axis research, and dietary strategies for bone health in postmenopausal conditions.