Mapping and cloning of a resistance gene for Albugo laibachii from the Arabidopsis accession HR-5

In a cross of HR-5xWs-2, resistance segregated 3:1 (R:S) in the F2 generation, suggesting a single gene/locus confers resistance. To map the gene responsible, 220 susceptible individuals from the F2 were collected and DNA extracted. This pooled "bulk susceptible" DNA was captured using the BrRenSeq v1 bait library to select for NLR encoding loci and sequenced on the MiSeq platform as one of a set of pooled barcoded samples (TGAC-ENQ-325/PRO647-repeat). To aid in identification of the gene responsible, stranded cDNA from HR-5 was later captured using the BrRenSeq v2 bait library and also sequenced on the MiSeq as one of a set of pooled barcoded samples (TGAC-ENQ-1124/PRO1067).