TaqMan probes used for the quantification of gene expression by qRT-PCR.
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Thermocycler parameters were 10 min denaturation at 95°C, followed by 40 cycles of 95°C for 15 sec and 60°C for 1 min. Values were interpolated into a standard curve to calculate absolute expression. These values were then divided by the expression of GAPDH in the same samples to correct for potential pipetting errors.
创建时间：
2015-12-02
