Functionalization of PEDOT:PSS for aptamer-based sensing of IL6 using organic electrochemical transistors

Explanation of included data for following publication:

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Functionalization of PEDOT:PSS for aptamer-based sensing of IL6 using organic electrochemical transistors

DOI: 10.1038/s44328-024-00007-w

Bernhard Burtscher (1), Chiara Diacci (1), Anatolii Makhinia (1,2), Marios Savvakis (1), Erik O Gabrielsson (1), Lothar Veith (3), Xianjie Liu (1), Xenofon Strakosas (1), Daniel T Simon (1)

1. Laboratory of Organic Electronics, Department of Science and Technology, Linköping University, 60174 Norrköping, Sweden

2. RISE Research Institutes of Sweden, Digital Systems, Smart, Hardware, Printed, Bio- and Organic Electronics, 60221 Norrköping, Sweden

3. Max Planck Institute for Polymer Research, Ackermannweg 10, 55128 Mainz, Germany

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The data is structured according to the first figure they appear in the manuscript.

Figure 2:

a) Transfer curves data as .txt at various stages of the functionalization process with information of

(Time / s; Gate Voltage / V; Drain Voltage / V; Drain Current / A; Gate Current / A; VR (measured voltage at the reference electrode) / V)

1) PEDOT:PSS

2) 4-ABA + PEDOT:PSS

3) Aptamer + 4-ABA + PEDOT:PSS

b) Measurement data of constant sensing experiments of either IL6 (6 meas.), BSA (3 meas.) or a control without aptamers (4ABA-ETA_Control_IL6-measurement) with explanation of when analyte was changed to which concentration.

c) Data of constant measurement of fully functionalized OECT in bovine plasma after incubation over night in bovine plasma and information regarding time and concentration of changes

Figure 3: Raw image files of fluorescent test of AL4083 PEDOT:PSS formulation with PLL-FITC as .nd2 files:

- 0.25% Gops with PLL-FITC (name: AL4083-Gops025_sample5_PLLFITC_FITC)

- 0.25% Gops without PLL-FITC (name: AL4083-Gops025_sample3_4ABA_noPLLFITC_FITC_5s)

- 1% Gops and 1% PSS with PLL-FITC (name: AL4083-Gops1_PSS1_sample4_PLLFITC_FITC)

- 1% Gops and 1% PSS without PLL-FITC (name: AL4083-Gops1_PSS1_sample3_4ABA_noPLLFITC_FITC_5s)

Figure 4: Frequency dependent OECT measurements of IL6 with PBS and different concentration of IL6. Each folder contains the nominal applied voltage (as .csv file) and the measurement voltage (in V) from the DAQ (as .tdms file in the ao1 column for each frequency). Data can then be processed with Python to fit the sine wave for each frequency to observe changes in the amplitude and phase.

Figure 5: Data taken from Figure 2b and Figure 4