five

Teosinte unigenes' sequences

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DataONE2016-03-01 更新2024-06-27 收录
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Due to the lack of available reference genome, clean reads from Zea may ssp. parviglumis and Zea mays ssp. mexicana were first de novo assembled into contigs using Trans-ABySS, which can use a multiple K-mer strategy to assemble the transcriptome. After that, transcriptome from different samples were pooled to reconstruct the transcriptome using CD-HIT-EST with a word length of 8 and an identity threshold ≥ 0.98.Sequentially, CAP3 was used to discard the redundancy transcripts, and the resulting contigs and singlets were combined to represent transcriptome of teosinte.
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2016-03-01
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