Pnldc1 Total RNA-seq of P10 Testis

PIWI-interacting RNAs(piRNAs) are required for transposon repression, de novo DNA methylation, epigenetic and post-transcriptional regulation of gene expression in the germline. A mist of piRNA biogenesis is the identification of the 3'-5' exnuclease that trimms the 3' end of piRNA intermediates to form mature piRNAs in mammals.Here, we show that mice deficient for PNLDC1, an evolutionarily conserved 3'-5'exonuclease, accumulate 3' untrimmed piRNA intermediates of 30~40nt. These 3' extended piRNA intermediates associate with PIWI proteins, but are impaired for function. Male Pnldc1 knockout mice display LINE1 derepression and are sterile owing to chimeric spermatogenetic arrest at spermatocyte and spermatid stages. Our findings illustrate the conserved role of mouse Pnldc1 in 3' trimming during piRNA maturation. However, since Pnldc1 knockout in mice creates phenotypes distinct from its reported interactive partner,Tdrkh,the panorama of participants in mammalian piRNA 3' end maturation may be more complex than previously speculated .There is the sequence of Pnldc1 Total RNA-seq of P10 Testis.PIWI互作RNA（PIWI-interacting RNAs，piRNAs）在生殖系的转座子抑制、从头DNA甲基化以及基因表达的表观遗传与转录后调控中发挥不可或缺的作用。piRNA生物发生领域尚存的一处认知盲区，是鉴定哺乳动物中可修剪piRNA前体3'端以生成成熟piRNAs的3'-5'核酸外切酶。本研究发现，进化保守的3'-5'核酸外切酶PNLDC1基因缺陷小鼠会积累30~40nt长度的未修剪piRNA前体。这些3'端延长的piRNA前体虽可与PIWI蛋白结合，但其功能却受到损伤。雄性Pnldc1基因敲除小鼠表现出LINE1去抑制现象，并因精母细胞与精子细胞阶段出现嵌合性生精阻滞而不育。本研究结果阐明了小鼠Pnldc1在piRNA成熟过程中3'修剪环节的保守功能。然而，由于小鼠Pnldc1基因敲除所产生的表型与其已报道的互作伴侣Tdrkh存在差异，哺乳动物piRNA 3'端成熟过程的参与因子全貌可能比此前推测的更为复杂。本数据集包含P10龄小鼠睾丸的Pnldc1总RNA测序数据。