Mouse embryonic stem cells (mESCs) differentiation towards ventral hindbrain neuronal cell types [d0-d3.5]

mESCs treated with retinoic acid (RA) and a Shh agonist (SAG) recapitulated ventral hindbrain development and produced a population of neural progenitors with a minor presence of other cell types. Overall design: The differentiation in mESCs was induced by RA and SAG in monolayer condition. The cells were collected every day and processed for RNA isolation and RNA-seq.