Vesicle movement prior to fusion with the plasma membrane.

Vesicle movements prior to fusion with the plasma membrane were determined in 3T3-L1 adipocytes (n = 4 cells) transfected with mCherry-IRAP-pHluorin and stimulated with 100 nM insulin.
aVesicle movements were scored as unknown when high local vesicle density or low mCherry fluorescence intensity made determination of movement difficult.
bLinear displacement of ∼1 µm or more was undetected prior to fusion of vesicle.