Some cases of discrepancies in classification between the newly defined lineages based on 12-loci MIRU-VNTRs and the Brudey's classification scheme in SpolDB4 (see footnote for extended explanation).

aAs an extended explanation, one may refer to the example of case F, where profile 1 corresponding to MIT310 (LAM7-TUR lineage on the basis of spoligotyping), and profile 2 corresponding to MIT430 (T3-ETH lineage on the basis of spoligotyping), both correspond to the Euro American-40 sublineage. Indeed, profile 1 with deletion of the block 20–24 and profile 2 with deletion of the block 10–19 could indicate a possible common ancestor with all spacers in positions 10 to 24 being present. As indicated by our IS6110AD-typing data (see text), this hypothetical ancestor would be harboring a copy of IS6110 between the spacers 19 and 20. Depending on the adjacent deletion located on the left or the right side of this IS6110 would result in the 2 different spoligotype patterns observed here, i.e., profile 1 or 2. Hence, albeit phylogenetically very close, these 2 isolates would be classified as LAM7-TUR and T3-ETH, in the Brudey's classification scheme in SpolDB4.bMIT; MIRU International Type according to the SITVITWEB database [5].