Efole et al. AJP-GI

<b>Figure S1.</b> <b>Protein expression analysis by western blot with stain-free normalization.</b><b> </b>(A) Western blot of tyrosine hydroxylase (TH) expression in the liver from the full cohort of mice fed chow or GAN diet for 12, 20 or 32 weeks. Stain-free gel images are shown below and were used for normalization of protein expression. (B) Western blot of α-smooth muscle actin (α-SMA) levels in livers of WT and <i>Adra1b</i>^LKO mice fed chow or GAN diet for 32 weeks, with corresponding stain-free gels used for normalization.<b>Figure S2. Biochemical and molecular characterization of WT and</b><b> </b><b><i>Adra1b</i></b>^{<strong>LKO</strong>}<b> </b><b>mice after 12 weeks of GAN diet.</b><b> </b>A) Plasma alanine aminotransferase (ALT), (B) aspartate aminotransferase (AST), (C) total bilirubin, and (D) albumin levels in WT and <i>Adra1b</i>^LKOafter 12 weeks of GAN diet. (E) Relative hepatic mRNA expression of <i>Tnf.</i><i> </i>(F) Relative hepatic mRNA expression of <i>Il1b.</i> (G) Relative hepatic mRNA expression of <i>Il10.</i> (H) Relative hepatic mRNA expression of <i>Ccl2.</i> (I) Relative hepatic mRNA expression of <i>Adgre1.</i> (J) Relative hepatic mRNA expression of <i>Cd68.</i> (K) Relative hepatic mRNA expression of <i>Arg1.</i> (L) Relative hepatic mRNA expression of <i>Tgfb.</i> (M) Relative hepatic mRNA expression of <i>Acta2.</i> (N) Relative hepatic mRNA expression of <i>Timp1</i>. Data are presented as mean ± SEM; n=8-9 mice per group.<br>