Hsa-miR-139-5p/HNRNPF axis modulates gene-transcripts balance in thyroid cancer cells

We explored alternative splicing events upon hsa-miR-139-5p/HNRNPF axis regulation in our cell system using the Multivariate Analysis of Transcript Splicing (rMATS) computational tool (https://doi.org/10.1073/pnas.1419161111). Differential analysis of major splicing outcomes (skipped exon, alternative 5'/3' splice, mutually exclusive exons and retained intron) revealed a total of 174 events significantly activated or repressed (FDR<0.05) upon HNRNPF regulation. Overall design: CAL62 isogenic cell lines expressing either mature hsa-miR-139-5p or a non-targeting control under a doxycycline inducible promoter were established. Cells were growth with or without doxycycline for 96 hours. The experiment was replicated 3 times and miRNA expression and HNRNPF mRNA/protein abundance quantified. HNRNPF was consistently decreases in doxycycline-treated hsa-miR-139-5p inducible cells.