mRNA expression in wild type and LRRK2 KO BMDM’s following PAM3CSK4 stimulation.

Bone marrow derived macrophages from LRRK2 knockout mice and wild type littermate controls were plated at a density of 1×106 in 2 ml of tissue culture media. Cells were treated with 1 µg/ml Pam3CSK4 24 h after plating and RNA collected at the indicated time points. Gene expression was normalized to GAPDH and the fold increase in mRNA expression compared to wild type macrophages at time 0 was calculated by the comparative Ct method (2-ddct). Data is presented as mean fold increase in expression ± S.E.M. Experiments were performed with n = 4 in duplicate.