five

Randomization in CyTOF (type 1 and type 2 data)

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http://flowrepository.org/id/FR-FCM-Z24G
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Show that randomization can negatively affect multivariate data analysis methods such as dimensionality reduction, clustering and network reconstruction algorithms. Conclusion: Randomization should be used only for visualization purposes, and never in conjunction with high-dimensional analytical tools. Notes: The fcs files refer to the same PBMC data after normalization and Type 1 and Type 2 randomization (see manuscript for details).
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2019-10-01
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