Long-read Transcriptome Sequencing from Multiple Tissues Provides Insights into Shikimic Acid and Anisatin Biosynthesis in Illicium anisatum

To get insight into biosynthesis of shikimic acids and neurotoxin anisantin, de novo transcriptome sequencing of Illicium satinum was performed. PacBio Iso-Seq data from different tissues of I. satinum, including leaf, stem, flower, and flower bud tissues, were generated and assembled. In addition, Illumina RNA-Seq data from those four tissues were produced, and expressions of unigenes, which were generated from PacBio Iso-Seq, were quantified. Besides the production of transcriptome sequencing data, whole-genome sequence data was produced and a complete chloroplast genome using the data was assembled.