Inhibition of SQLE or FDFT1 in RBE cells by shRNA, or RBE cells treated with lycorine hydrochloride

High-throughput sequencing was performed to analyze gene expression profiles of RBE cells after the the inhibition of SQLE or FDFT1 by lentiviruses-mediated shRNA, or RBE cells treated with lycorine hydrochloride. RBE cells were treated for 24 hours with either DMSO, or lycorine hydrochloride (1μM), and cells were collected from each treatment group to perform high-throughput sequencing and mRNA expression profile data analyzing. For the inhibition of SQLE or FDFT1 in RBE cells, the expression of SQLE or FDFT1 was knocked down by lentiviruses-mediated shRNA, the control and SQLE or FDFT1 -deficient RBE cells were subjected to high-throughput sequencing and mRNA expression profile data analyzing.