Characterization of E11.5 pre-HSCs and E12.5 & E14.5 HSCs to understand their transition using scRNA-seq

In oroder to understand the maturation process of pre-HSCs to HSC, we conducted scRNA-seq using E11.5pre-HSCs and E12.5&E14.5 LT_HSC populations. Pseudotime analysis and velocity analysis show the progression of pre-HSC to mature HSCs and also divergent point of lineage trajectory. Characterization of E11.5 pre-HSCs and E12.5 & E14.5 HSCs to understand their transition using scRNA-seq. Each cell was single cell sorted into 96 well plate (1 cell/well) and RNA was extracted and converted into cDNA using SAMRT-Seq Single cell kit (Takara)