454 sequencing of reduced representation libraries to discover single-nucleotide polymorphisms (SNPs) in Megabunus harvestmen

The goal of this study was to obtain nuclear sequences from a reduced representation of the genome of four harvestmen species suitable for the development of nuclear sequence markers. These markers will be useful for research on molecular phylogenetics and species delimitation. We sampled four adult harvestmen within the family Phalangiidae, all organisms lacking a sequenced genome: Rilaena triangularis and three specimens of the genus Megabunus (M. armatus, M. vignai, M. lesserti). RRL construction used a modified AFLP method (Leroux et al. 2010). Size selection of the amplicons was performed by electrophoretic separation and cuts from denaturing agarose gels.