Figure_5_replicates

<em>(G-I) Viral RNA was extracted from viral stocks (G), Xrn1 knock out A549 cells 16 hours post-infection (H), or 48-hour supernatants from infected MDCK cells (H). Viruses analyzed: </em> <em>WT PR8 and PR8-PA(∆X) strains with no insert, or PR8 with WT or mutant </em>STOML2<em> sequences inserted in the WT PR8 or PR8-PA(∆X) background. Mutant </em>STOML2<em> sequences contain the GCUG to UAGC mutation preventing PA-X cleavage. Viral RNA was reverse-transcribed and PCR-amplified using primers shown as black arrows in F-G to determine whether the </em>STOML2<em> sequences were retained.</em> <em>R# --&gt; replicate number; LG### --&gt; experiment number</em> <em>.sgd are the original source files obtained with the Syngene Imager</em>