Figure S1 - A Protective and Safe Intranasal RSV Vaccine Based on a Recombinant Prefusion-Like Form of the F Protein Bound to Bacterium-Like Particles

Sequences of the GCN4 trimerization domain and the tags used. The carboxy-terminal residues of the RSV F protein ectodomain are shown in blue. The triple Strep-tagII (ST3; italic, green), GCN4 trimerization domain (red, bold) and the LysM domain (underlined, including LysM linker sequences) are indicated. F proteins containing ST3 were used in the in vitro analysis, while the LysM domain-containing proteins lacking the ST3 tag were used for BLP binding. (DOCX)