LncRNA NONMMUT104330.1-miR-709-DDX58 axis mediates enoxaparin sodium bone cement to regulate M2 polarization of macrophages and play an anti-inflammatory immunomodulatory role

PMMA bone cement is biologically inert and exhibits poor biological activity. Following implantation, it generates bone cement particles, which can easily induce tissue inflammation around the implant . Compared with PMMA bone cement, ES-PMMA bone cement had anti-inflammatory effect by inducing M2 polarization of macrophages. RNA sequencing was used to explore the underlying molecular mechanisms. To explore the molecular basis of the anti-inflammatory effect of ES-PMMA bone cement. We prepared PMMA bone cement and ES-PMMA bone cement sample extract medium. RAW264.7 cells were induced by LPS for 24 hours, and then the culture medium was changed to different bone cement extract medium for further culture. RAW264.7 cells treated with different bone cement extracts were collected for RNA sequencing to screen out differentially expressed genes.