Fabrication of an Asymmetric PCL-HAP/CS Bilayer Composite Membrane with Dual Osteogenic and Fibrogenic Functions for Guided Bone Regeneration

This project contains experimental data and figures associated with the paper <i>"Fabrication of an Asymmetric PCL-HAP/CS Bilayer Composite Membrane with Dual Osteogenic and Fibrogenic Functions for Guided Bone Regeneration" </i>(under review). For any additional inquiries, please direct correspondence to: <b>Email</b>: [e-mail] 3 Dispersion of hydroxyapatite in ethanolFigure 4 Infrared spectra comparison before and after HAP modificationFigure 5 XRD spectra of three kinds of filmFigure 6 Infrared spectra of three kinds of membrane materialsFigure 7 Characterization and cross sections with different membrane materials. (A. PCL-HAP Membrane; B.CS Membrane; C. PCL-HAP/CS Membrane; D.PCL-HAP/CS Membrane Section)Figure 8 Hydrophilic test figure three kinds of membrane materialsFigure 9 Curves of three kinds of membrane DSCFigure 10 The TG curves of three kinds of membrane materialsFigure 11 Three kinds of membrane materials of two cell culture photos. A. CS Film(Fibroblast cells L929); B, CS Film( Osteoblast cells MC3T3-E1); C. PCL-HAP Film(Fibroblast cells L929); D, PCL-HAP Film(Osteoblast cells MC3T3-E1); E. PCL-HAP/CS Film(Fibroblast cells L929); F, PCL-HAP/CS Film(Osteoblast cells MC3T3-E1);Figure 12 These fluorescence images illustrate the variations in cell morphology and proliferation on different membranes A, CS Film(Osteoblast cells MC3T3-E1); B, PCL-HAP Film(Osteoblast cells MC3T3-E1); C, PCL-HAP/CS Film(Osteoblast cells MC3T3-E1); D. CS Film(Fibroblast cells L929); E. PCL-HAP Film(Fibroblast cells L929); F. PCL-HAP/CS Film(Fibroblast cells L929);Figure 13 Comparison in the adhesion and proliferation of the fiber cell and osteoblasts membrane surfaceFigure 14 Scan-based modeling images of the PCL-HAP/CS composite membrane on new bone formation in rat skull defects. A, D: Blank control group; B, E: Chitosan (CS) layer in contact with the skull; C, F: PCL-HAP layer in contact with the skull Panels A, B, and C correspond to 6 weeks post-implantation, while panels D, E, and F represent 12 weeks post-implantation.