Gene expression data of Lsd1fl/fl and Lsd1fl/fl EpoRCre CD71_high / c-Kit_high pro-erythroblasts.

We discovered that mice lacking Lsd1 in the erythroid lineage die in utero of a lethal anemia around embryonic day E13.5. Lsd1 knockout embryos displayed an increase in CD71_high c-Kit_high pro-erythroblasts, followed by a drastic reduction of later maturation stages. To determine the genes altered by Lsd1-loss, CD71_high c-Kit_high pro-erythroblasts from Lsd1fl/fl and Lsd1fl/fl EpoRCre mice were FACS-purified to be analyzed by gene expression profiling. Primary CD71high c-Kit high pro-erythroblasts were isolated by FACS-sorting from fetal livers of E13.5 Lsd1fl/fl and Lsd1fl/fl EpoRCre embryos. Total RNA from two biological replicates per genotype was extracted and used to hybridize to Affymetrix expression arrays using the Mouse Genome 430A array platform.本研究发现，红系谱系中缺失Lsd1的小鼠会在胚胎发育第13.5天（E13.5）左右因致死性贫血死于子宫内。Lsd1敲除胚胎的CD71高表达、c-Kit高表达的前成红细胞比例升高，随后晚期成熟阶段的细胞数量急剧减少。为明确Lsd1缺失所改变的基因，我们从Lsd1fl/fl及Lsd1fl/fl EpoRCre小鼠中分离CD71高表达、c-Kit高表达的前成红细胞，经荧光激活细胞分选（FACS）纯化后进行基因表达谱分析。我们从E13.5天Lsd1fl/fl及Lsd1fl/fl EpoRCre胚胎的胎肝中，通过FACS分选分离得到原代CD71高表达、c-Kit高表达的前成红细胞。提取每种基因型的两份生物学重复样本的总RNA，采用小鼠基因组430A芯片平台与Affymetrix表达芯片进行杂交。