Figure S3

*Note* data organized into .Zip folders we suggest to maintain format when downloading. Figure S3a -Coommassie stained SDS-PAGE gels of purified Bcl-XL, mCerulean3 and Venus proteins Figure S3b -Lifetime data acquired for purified mCerulean3 and Venus on ISS-Alba. Original VistaVision files included as well as exported lifetime histogram. Figure S3c \"Figure S3c Tecan data.xlsx\"= Original Excitation/Emission scan on Tecan Fluorimeter. Figure S3d Example exported results from qF3 Replicate Analysis step 2. Date acquired= 20190408. Figure S3e Check TCSPC counts by exciting each fluorophore independently. Figure S3f mc3_bleedthrough_into_spectal_channel Figure S3g -FRET histogram data for each construct -Phasor plot data for each construct -MATLAB Figure, Top & Side views of 3D dataset used to determine G factor (image data available upon request) Figure S3h Source data for 3 replicates > combined to generate histograms for mCerulean3-fusion protein expression distribution in BMK-DKO. Figure S3i Binned binding curve data, filtered by mCerulean3 level. Several ranges sampled.