Oxoeicosanoid signaling promotes early antimicrobial defense in zebrafish (Danio rerio)

RNAseq was performed on hcar1-4+/+ and hcar1-4-/- zebrafish larvae with (2 h and 4 h) or without (0 h) Pseudomonas aeruginosa (PA) ear infection. The transcriptome profile generated here reveals PA14 infection-induced differential gene expression patterns between hcar1-4-/- and hcar1-4+/+ siblings. Overall design: Pseudomonas aeruginosa resuspended in standard E3 was injected into the otic vesicle of hcar1-4+/+ and hcar1-4-/- zebrafish larvae. Uninjected zebrafish larvae served as control. Total RNA was extracted before or after 2 hours and 4 hours of infection, and processed to following sequencing.