Intra-assay variation experiment for DNA ploidy with FxCycle Violet and simulatneous surface immunophenotyping

To study the intra-assay variation in DNA ploidy analysis using FxCycle Violet and simultaneous multicolor immunophenotyping
Conclusion:
The inta-assay variation of DNA ploidy analysis using FxCycle Violet and simultaneous multi-color immunophenotyping is almost negligible.
Notes:
In this experiment, a peripheral blood sample is analyzed for intra-assay variation in DNA index of the cells studied using FxCycle Violet. The same sample is processed and acquired ten times and DNA index of CD19+ cells is calculated. Sample is acquired on FACS Canto-II and daily QC was performed using CST beads. a generic compensation was applied during acquisition and tube specific compensation is done during analysis of data. approximately 10000-20000 events were acquired at low rate. Sample was processed within 12 hours of collection. Timing of fixation was strictly followed.